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For Hoecst staining, tissue was washed in PBS, blocked in 2% BSA, 0.1% Triton X-100, washed and stained using Hoecst (Thermo Fisher 1:2000)
A few of my favorite fancier flymphs right now come from the brain of Brian Silvey: Silvey's Bead Head Prime Time Pupa (or at Slide Inn) and Silvey's Caddis Pupa
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The ported aluminum spool reduces weight while the 90mm aluminum handle with molded oversized knobs provides excellent torque
Also included is a uniquely designed power filter with SmartClean Technology that makes essential water changes quick, fast and easy to do in less than 2 minutes
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